 |
 |
 |
 |
 |
Calman Prussin
>----------
>From: Janek Brozek
>Sent: Tuesday, December 3, 1996 13:04
>To: Cytometry Mailing List
>Subject: Re: PE-Cy5 or PER-CP
>
>
>As far as I know there is no commercial source of anti-human or anti-mouse
>immunoglobulins directly conjugated to PE-Cy5 or PerCP. There are ways to
>bypass it, though.
>1. Get the biotinylated antibody and the streptavidin conjugated PerCP from
>Becton Dickinson, or streptavidin-PE-Cy5 from, say DAKO. The best source of
>biotinylated goat anti -human/-mouse MoAbs is Jackson ImmunoResearch.
>
>2.Get those anti-immunoglobulin MoAbs conjugated to FITC or PE and the other
>ones you want to use (in a 3 color setup) conjugated to PerCP or PE-Cy5.
>This idea does not work if you need ALL of your MoAbs (FL1,2,3) directed
>against immunoglobulins, of course.
>
>Hope this helps a bit,
>
>Janek
>
>--------------------------
>mmbrozek@cyf-kr.edu.pl
>http://www.cyf-kr.edu.pl/~mmbrozek
>
>
|
|
|
|
|
|
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web