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Does anyone have any recommendations about which manufacturer's kit does a
good job? I have experienced variable quality in purchased kits, and
would like to hear from someone who has actually used these before
investing the money. Thanks!
Rosemarie
Rosemarie,
I have had pretty good luck with the mouse monoclonal anti-BrdU from Becton
Dickinson (both unconjugated-cat. # 347580 and conjugated to FITC-cat. #
347583). The package insert includes a method for staining incorporating DNA
denaturation with HCl. I used this method with CHO cells. This is not a kit,
but all the reagents are readily available. Hope this helps.
Linda McGarrity, FDA, Little Rock, Arkansas has made some modifications to this
method and was kind enough to help me with this. If you're interested in the
modified procedure, please feel free to contact me.
Connie Wagner
Monsanto Environmental Health Lab
645 S. Newstead Ave.
St. Louis, MO 63110
(314) 694-7971
email: cawagn@ccmail.monsanto.com
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web