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Dear Dr. Wang,
The problem you are having labeling with pulsed BrdU and surface markers at
the same time is not unique to your laboratory. However, there is a
solution. I refer you a new methodology called SBIP ( Strand Break Induced
Photolysis). The methodology is outlined in the paper by Li, X.
Darzynkiewicz, Z: Labeling DNA strand breaks with BrdUTP. Detection of
apoptosis and cell proliferion, Cell Proliferation, 28, pp571-579, 1995.
Phoenix Flow Systems will be releasing a kit based on this methodolgy
August 15.
Regards,
C. Kevin Becker
Phoenix Flow Systems, Inc.
11575 Sorrento Valley Rd. #208
San Diego. CA, 92121 USA
(619) 453-5095 Fax (619) 259-5268
info@phnxflow.com
> From: Xiao-Tian Wang (Medical Education) <wangx01@doc.mssm.edu>
> To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
> Subject: Question about Brdu double staining
> Date: Wednesday, July 02, 1997 5:46 PM
>
>
>
> Dear collegues:
>
> In my laboratory work, I attempt to double stain the cells with Brdu (for
> proliferation assay) and a cell surface marker. The cells I am working
> with are the cell mixture of intestine epithelial cells and T cells. I
> don't know if anybody has tried to do the similar things. It seems a
> little hard to do since the anti-Brdu Ab has to enter the nucleus while
> the other Ab has to bind to the cell surface moleculels. I would
> appreciate any input. My email address is wangx01@doc.mssm.edu
>
> Tian Wang
> Clinical Immunology
> Mt. Sinai School of Medicine
> Tel:(212)-824-7466
>
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web