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Title: Background-reducing compounds for probe-mediated in-situ fluorimetric
assays
Author: Cubbage, Michael Lee; Ju, Shyh Chen; Prashad, Nagindra; Weber, William
Dugald; Bresser, Joel
Source: PCT Int. Appl. 95 19450 48 pp.
Year: 1995
Where Held:
Info Category: SUCL15
Subject:
Company: Aprogenex, Inc.USA
Country:
Keywords: in situ fluorometry background reducing dye:fluorescent staining
background reducing dye:hybridization fluorescent in situ background redn:
Issue: 9508
Document Type:
Language: Eng
Origin:
Text:
Assays for target mols. (e.g., viral nucleic acids, human genes, cellular or
viral antigens) in biol. cells and viruses are disclosed wherein the use of
appropriate light-absorbing mols. (e.g., Evans Blue, sulforhodamine 101, trypan
blue, etc.) at an appropriate stage of the assay procedure leads to decreased
nonspecific emission of light and/or decreased autofluorescence.
Gerhard Nebe-v.Caron
Unilever Research, Colworth,
Sharnbrook, Bedfordshire
GB - MK44 1LQ
Tel: +44(0)1234-222066
FAX: +44(0)1234-222344
gerhard.nebe-von-caron@unilever.com
______________________________ Reply Separator _________________________________
Subject: Autofluorescence Quenching
Author: wlauzon@uottawa.ca at INTERNET
Date: 23/01/97 00:34
Hello,
I've been lurking around this E-mail list for about a month now enjoying
the discussions and the information being exchanged.
I have recently heard of a technique used in flow based in situ PCR
where the autofluorescence is greatly reduced by the addition of trypan
blue. It was just a casual reference in a talk and I was unable to find
anything more about it from the speaker.
I was wondering if there was anyone out there with experience in this
technique who could provide a protocol or a good reference for one.
While I have your ear, I would also appreciate any information that you
may have concerning epitope tagging reagents: anti-CDx-DIG/anti-DIG-PE
etc. If there are commercially available reagents I would be eager to
find out the sources.
Thank you for your time
Wallace Lauzon
Dept. Microbiology and Immunology
University of Ottawa
451 Smyth Rd
Ottawa, Canada
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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