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Well, you are absolutely right with this, but the authors of the =
cited paper
themselves wrote about this confusion, so I'm still a bit skeptical. =
What
if I gated out aggregates and right in these aggregates were a higher =
percentage
of vital targets (i.e. not yet killed targets but targets adherent to =
effectors)
in comparison to the singlet cells (i.e. more already killed targets =
not anymore
adherent to effectors), well okay, I'm probably a bit too skeptical.
[...]
>Finally, if you really think you will have dead effectors you could mark =
that
>population also before the co-incubation or by post-staining with a =
effector
>specific mAb.
Yes, by using a marker mAb you should be able to clarify the =
situation.
Thank you very much for your reply.
-- Andreas Schr=F6der
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
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