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Mike Fox
On Mon, 16 Jun 97 11:56:27 -0600 CHELACK@admin3.usask.ca wrote:
>
> What kind of methods are being used to sort viable X and sperm in particular
> bull sperm. A graduate student has brought me some older literature
> describing sorting on differences in DNA content using specialized adaptors
> to maintain consistent orientation of the sperm as they pass through the flow
> cell. Is it possible to use FITC oligos to specific regions of the Y
> chromosome to accomplish this? I have sent the graduate student back to search
> out more on this possibility, but am wondering if anyone out there is already
> doing this or has found it to be impossible, or is using another even more
> elegant method? The graduate student is quite keen and quite green so I am
> trying to make sure he isn't biting off more than he can chew.
>
>
> regards
>
>
> BJC - Brian
Mike Fox
Chairman, Cell and Molecular Biology Graduate Program
Dept. of Radiological Health Sciences mfox@cvmbs.colostate.edu
Colorado State University Ph: (970)491-7618
Ft. Collins, CO 80523 USA Fax:(970)491-0623
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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