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The tricks are simply
-to wash thoroughly (at least 2x) with PBS first to remove culture medium
-to be strict about the time and temp which you allow exposure to trypsin:
usually <3mins, at RTemp <24 Celsius in our hands
-we use 0.05% or 0.02% in PBS
If you need any more info, email me.
regards,
Nick
>From: ans033@abdn.ac.uk
>Subject: adherant cells
>To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
>Date: Thu, 12 Jun 1997 15:57:43 +0100 (BST)
>X-Mailer: ELM [version 2.4 PL21]
>X-PMFLAGS: 33554560 0
>X-UIDL: 22cabc4bea1f1008bab3a51f54a533be
>
>
>Could anyone suggest a method of removing adherant cell lines from culture
>vessels which does not remove surface adhesion molecules? At present i am
>studying surface cell adhesion molecules expression on human umbilical vein
>endothelial cells using flow cytometry.
>i would appreciate any suggestions.
>thanks
>Morgan
>
>
Nicholas J.C. King, M.B. Ch.B., Ph.D.
Department of Pathology
Blackburn Building, D06
University of Sydney
New South Wales 2006
Australia.
nickk@med.su.oz.au ,-_|\
nickk@pathology.usyd.edu.au / \
Ph. 61 2 351 4553 \_,-._* <- USYD
FAX: 61 2 351 3429 v
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
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If you have any comments please direct them to
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