Purdue Cytometry CD-ROM Volume 3
Clinical Immunophenotyping

Immunophenotyping, the characterization of cell subpopulations based on their surface markers, is not only an important part of immunological research but also has significant clinical relevance for the diagnosis and management of many disease processes. The combination of monoclonal antibodies and flow cytometry provides a powerful tool for the phenotypic analysis of human white blood cell subsets. The diagnosis of and prognosis for leukemias and lymphomas is perhaps the most common application of clinical immunophenotyping. Others include CD4:CD8 ratio determination for monitoring AIDS patients, crossmatching for organ transplantation, and monitoring for post-transplant rejection episodes. We have included in this section some clinical methods and interesting case studies, which we hope you will find useful.

1. Clinical Methods
   • Immunophenotypic Analysis of T-Cell Depleted Bone Marrow Cells  L. S. Lamb
     
   • Immunofluorescent staining of intracellular cytokines for flow cytometric analysis.   G. Basso
   • A multiparametric approach to immunophenotyping.  C. Ortolani
   • Phenotypic and functional characterization of tumor infiltration lymphocytes:
     detection of an oligoclonal population with peculiar features.  S. Bruno, et al.
   • Flow cytometric immunophenotyping of HIV infected subjects and quality control.  A. Kunkl
   • A Strategy for B Cell Minimal Residual Disease Detection  P. I. Fukushima et al.
2. Leukemia and Lymphoma
3. Other Blood Diseases  W. Sharp
4. Microscope Images  W. Sharp
5. Rare Event Detection - Clonal Plasma Cells  M. Woda
6. A 3-D Animated Movie of Flow Data from Peripheral Blood Lymphocytes  S.A. Schwartz et al.
7. References   K. Ragheb
8. Selected Discussion from the Email Archive
   • Discussion of Clinical Flow Issues
   • Discussion of Cytometry Software
   • Discussion of Immunophenotyping
   • Discussion of Microbiological Applications of Cytometry
   • Discussion of Flow Cytometry Instruments
   • Discussion of Prep and Fix for Cytometry
   • Discussion of Reagents for Prep and Fix
   • Discussion of General Flow Cytometry Techniques
   • Discussion of Computer Hardware for Cytometry

Note: These data have been provided by colleagues worldwide. Appropriate acknowledgement should be made when material is used.

CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(765) 494-0757; FAX (765) 494-0517; Web http://www.cyto.purdue.edu, EMAIL cdrom3@flowcyt.cyto.purdue.edu