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[Introduction to STEP]
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Plant Cell Studies
ENEA C.R. Casaccia
Biochemistry, Biophysics and Molecular Biology Division
Via Anguillarese, 301
00060 Roma, IT
Laboratory Profile
Director: Dr. Sergio Lucretti
1.Facilities available: 200 m 2 facility containing instrumentation lab, molecualr and cellular biology labs, plant tissue culture facilities, growth chambers,
2. Instrumentation:
Flow cytometry: Becton Dickinson FACStar PLUS dual laser (argon 5W and 2W visible); also available Partec PasII(Hg lamp flow-cytometer).
Other major items: Microspectrofluorimeter Leltz-SP for analysis of light absorption and fluorescent emission from microscopic specimens; normal and inverted microscopes equiped with fluorescence; also available a BioRad MCR 600 confocal microscope.
Computer and Analytical: Al flow-cytometric data are compatible with MS-Dos and HP Pascal 3.2-based instruments; non cytometric software expertise on MacIntosh computers.
3. Specialization areas: Flow-karyotyping and sorting of plant chromosomes; Mono- and biparametric cellcycle analysis of in vivo and in vitro explants; Plant protoplast isolation and sorting; Somatic hybridization of plant protoplasts; Direct mcroencaps ulation of sorted particles by flow cytometry.
4. Training Opportunities: Cell-cycle synchronization of organs and cell suspensions for chromosome and nuclei isolation; Plant cell-cycle analysis by BrdU and immunolabelling; In vitro plant tissue culture; Somatic hybridization of protoplasts and plant regeneration; Plant transformation. Our lab has extensive experience on DNA ploidy evaluation from different explant sources and sorting of protoplasts under biotic stress conditions.
5. Special courses offered: BD FACStar PLUS usage; Plant protoplast isolation, fusion and plant regeneration in Citrus,
6. Graduate Program: None.
7. Local Accomodations: In Rome, near ENEA headquarters, arrangement with hotels as low as 70 $/single room.
8. Transportation: Local airport is Rome International Airport (Leonardo da Vinci). Several free shuttle bus connections from ENEA headquarters to C.R. Cassacia - research center 30 km north-west of Rome.
9. Cost of Internships: base rate of about 400 $ per week will cover most of laboratory cost and flow cytometry, depending on what studies are required. The period for internships must be arranged directly.
Dr. Sergio Lucretti
ENEA C.R. Casaccia
Biochemistry, Biophysics and Molecular Biology Division
Via Anguillarese, 301
00060 Roma, ITALIA
Phone: (39) 6 3048 3191
FAX: (39) 6 3048 6545
Telex: 613296 ENEACA I
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(765) 494-0757; FAX (765) 494-0517; Web http://www.cyto.purdue.edu, EMAIL cdrom3@flowcyt.cyto.purdue.edu