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Michigan Cancer Foundation 110 East Warren Detroit, MI 48201
1. Facilities: Labs are located in Michigan Cancer Foundation, a Wayne State University Medical School affiliate. Analytical Cytometry facility includes instrumentation labs, computer facility, tissue culture facility, space for visiting scientists.
2. Instrumentation:
Flow cytometry: Becton-Dickinson FACStar cell sorter with innova 90 argon laser; EPICS 753 dual laser cell sorter with 2 innova 90 argon lasers and CR559 dye (Rhodamine 6G) laser. Also available is a Becton-Dickinson FACScan 5-parameter flow cytometer.
Other major items: Olympus and Leitz phase/fluorescence microscopes equipped with CCD, Video, and standard camera systems. Perkin Elmer LS-3B fluorescence spectrometer, SLM-Aminco 8000C dual beam fluorescence spectrometer, Millipore Cytofluor 2300 multi-position plate reader, Zeineh Scanning Video Densitometry system.
Data acquisition and analysis: HP 9000 series systems, running Consort30, LYSYS II, and Paint-A-Gate Plus (automatic 3-color analysis with spreadsheet-compatible output); MDADS and EASY2 systems; several MS-DOS systems offering MODFIT and ISOCONTOUR (Verity), Elite (Coulter), MultiCycle (Phoenix), along with other graphics and statistics programs (CorelDraw, Picture Publisher, Sigma PLOT, InSTAT QuattroPRO, and Excel).
3. Specialization Areas: Extensive experience in measurement of functional parameters of drug resistance via dye/drug efflux assay in both solid and hematopoietic tissues. Modulation of efflux-related resistance with efflux blocking agents. Immunofluorescent determination of p-glycoprotein expression (C219, MRK16). Measurement of other mechanisms of resistance; DNA topoisomerase II, bc1-2, and MDR1 expression; glutathione levels. Immunofluorescence and DNA content analysis expertise.
4. Special Opportunities: None at this time.
5. Special Courses Offered: Drug efflux modulation studies; FACScan instrument operation; DNA content analysis; procedures for labeling p-glycoprotein by immunofluorescence and immunohistochemistry.
6. Graduate Program: None at this time.
7. Local Accommodations: Hotel St. Regis is within 2 miles.
8. Transportation: Local: Detroit City Airport. Major airport: Detroit Metropolitan International.
9. Cost of Internship: $500.00 per week (negotiable).
10. Communication:
Awtar Krishan, Ph.D., Scientific Director Michigan Cancer Foundation 110 East Warren Detroit, MI 48201 Phone: (313) 833-0715 x-306 FAX: (313) 832-0531 E-mail: awtark@mcf.roc.wayne.edu
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(765) 494-0757; FAX (765) 494-0517; Web http://www.cyto.purdue.edu, EMAIL cdrom3@flowcyt.cyto.purdue.edu