Re: MACS CELL SORTING

dsg4@psu.edu
Thu, 31 Aug 1995 09:01:55 -0400

Are CD15S (neutrophils) and CD62L (L-selectin) available directly conjugated
to a fluorochrome?
Are there any other surface markers for neutrophils only?

One of the reasons I have heard that people use whole blood when they label
cell surface antigens is so that the do not lose any populations due to
lysis of RBC's. However, don't these procedures all include a lysis step at
the end anyway? Have I missed the point?
Are there other procedures I should know about?

Thanks!
Deb G.

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