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The blast cells which appear during acute transformation of CML have
been charaterized as resembling lymphoblasts (ie. CD10+/CD19+). At other
times the bone marrow phenotype can be quite heterogeneous. In my
experience you could expect to see a wide variety of myeloid precursors
and the proportions of each would be variable over time. I usually
advised our hematologists against monitoring CML patients since it was
difficult to make sense of the dysplastic changes evolving. In an
individual undergoing blast transformation in the setting of a
long-standing myelodysplastic disorder I think the flow would be very
useful since therapeutic decisions may depend on it.
As to your questions about culturing cells, I can't offer any advice.
Good luck.
Michael Weaver
6731 Steveston HIghway
Richmond, B.C.,
V7E 2L2
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