Tdt Negative Lymphoblasts
Lucille H Kimura (lucille_h.kimura@tamc.chcs.amedd.army.mil)
Fri, 11 Apr 1997 17:19:31 -1000
We recently ran phenotyping on bone marrow cells from a 21-year old patient
having cells which appeared morphologically to be lymphoblasts. The cells
were CD2+, CD3+, CD7+, CD45+, DR-, CD5- and Tdt-. This is the first case we
have seen of Tdt negative lymphoblasts and were wondering how often this has
been observed by others. Our cases of pediatric ALLs are usually CD45- and
Tdt+, suggesting that our current case may have a slightly more mature
phenotype although presenting clinically as an ALL.
A second unrelated question is how to achieve maximal viable cell recovery on
anaplastic large cell lymphomas. Although we usually recover sufficient
numbers of viable lymphoid cells on fresh lymph node biopsies, we
occasionally will have a case in which the cell recovery is extremely low
even though the original tissue sample appears adequate. We routinely gently
mince the tissues on a fine mesh screen and wash the cells with HBSS before
staining. Any contaminating red cells are lysed with ammonium chloride before
staining. Have others run into problems with lymphoma cells that are
extremely fragile? Thank you in advance for your suggestions.
Lucy Kimura
Department of Pathology
Tripler Army Medical Center
TAMC, Hawaii 96859-5000
Lucille_H.Kimura@TAMC.CHCS.AMEDD.ARMY.MIL
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