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I am setting up an assay to screen for the expression of CD18,
11a,11b and 11c on neutrophils; taking mean peak intensity of
resting versus stimulated cells.
Is it possible that some of you have used Ca ionophore for this
purpose and could advise me as to an appropriate concentration fro
stimulation ( or advise a reference).
Many thanks
Jane Hughes
Immunology Section
Dept Paediatrics
Red Cross Children's Hospital
Rondebosch, Cape Town, South Africa
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web