Autofluorescence
Abby Allen (allen@cbr.med.harvard.edu)
Mon, 28 Apr 1997 10:23:16 -0400
Hello!
I have been asked about some flow problems with an LCL (lymphoblastic
cell lines). Specifically, even cells unstained show a bright diagnal
region equidistant from both the FL1 and FL2 axes rather than the usual
negative cells. I am not familiar with LCLs and I have never seen this
degree of autofluorescence. The investigator is presently checking on
the blocking steps and the media the cells were in. I was wondering if
anyone else has had this problem and a suggestion for this
investigator?
As always thanks in advance for your advice!
Abby
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Abby Allen
Center for Blood Research
800 Huntington Ave.
Boston, MA 02115
allen@cbr.med.harvard.edu
**********************************************************************
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
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