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CYTOMETRY COURSE and WORKSHOP
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CONFOCAL IMAGING, FLOW CYTOMETRY, AND IMAGE ANALYSIS
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Jagiellonian University
Krakow, 10-13 June 1997
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For detailed information about this Course, please check:
http://www.if.uj.edu.pl/~ubdobruc or
http://helios.mol.uj.edu.pl
or the mirror in USA:
http://www.cyto.purdue.edu/mirrors/dobrucki/index.html
On-line registration:
http://helios.mol.uj.edu.pl/kurs/app.html
or write to: dobrucki@mol.mol.uj.edu.pl
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Just a reminder - the lectures are free of charge but advance
registration is required.
Due to an overwhelming number of applicants we decided
to create an additional group (5 students) for laboratory
sessions/tutorials. If you wish to register or send your student
for a Course - MAKE UP YOUR MIND NOW.
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I enclose below an updated list of Course teachers as well as
lectures, laboratory sessions and tutorials:
TEACHERS:
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Prof. G.J.Brakenhoff, University of Amsterdam, The Netherlands
Prof. A.Cossarizza, University of Modena, Italy
Prof. Z.Darzynkiewicz, New York Medical College, New York, USA
Dr G.Durack, University of Illinois at Urbana-Champaign, USA
Prof. W.G=F6hde, Westf=E4lische Wilhelms-Universit=E4t, Germany
Prof. J.Kawiak, Medical Center for Postgraduate Training, Warsaw, Poland
Dr H. Malak, University of Maryland, Baltimore, USA
Dr N. Opitz, Max-Planck-Institut f=FCr Molekulare Physiologie, Dortmund, G=
ermany
Prof. J.Pryjma, Jagiellonian University, Krakow, Poland
Prof. P.Robinson, Purdue University Cytometry Laboratories, USA
Prof. F.Sansonetty, Hospital de Sao Joao, Porto, Portugal
Prof. G.K.Valet, Max-Planck-Institut f=FCr Biochemie, Martinsried, Germany=
Dr J.V.Watson, Addenbrooke's Hospital, Cambridge, England
Dr N.White, University of Oxford, England
LECTURES and LABORATORY SESSIONS:
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F L O W C Y T O M E T R Y - L E C T U R E S
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J.P.Robinson Flow Cytometry and Confocal Microscopy: Similar
Measurement Systems but Different Solutions
Z.Darzynkiewicz
Cytometric Methods of Detection of Apoptosis and
Accidental Cell Death (Necrosis)
Z.Darzynkiewicz
Cytometry of Cyclins and CDKs Inhibitors in Cell
Cycle Analysis
G.Valet Cell Function in Flow Cytometry
J.V.Watson Drug Resistance Assays in Flow Cytometry
J.Pryjma Detection of Tumour Cells in Peripheral Blood
W.G=F6hde High Resolution One- and Multiparameter FCM
F L O W C Y T O M E T R Y -
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L A B O R A T O R Y S E S S I O N S and T U T O R I A L S
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1. Introduction to Flow Cytometry - DNA analysis, Phenotyping,
Cytotoxicity
2. Cell Function (Mitochondrial Activity) by Flow and Confocal
3. Time and dynamic cellular events
4. Data Analysis in Flow Cytometry
5. Multiparameter Data Analysis
6. Discussion Session of General Problems in Flow Cytometry -
Sorting, Electronics, Fluidics, Optics
7. Use of Internet in Cytometry (tutorial)
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The following laboratory sessions and tutorials will
be organized if sufficient number of participants express interest:
Measurements of Oxidative Metabolism by Flow
Platelet Activation
Measurement of nuclear antigens
Standardized Multiparameter Data Classification (SMDC) in Medicine
C O N F O C A L M I C R O S C O P Y - L E C T U R E S
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F. Brakenhoff Confocal principle, imaging modes and
optimalisation of confocal imaging
N. White Multidimensional confocal microscopy of intact,
living tissues
F. Brakenhoff Confocal contrast modes, photon budgets and real
time imaging
F. Brakenhoff New confocal techniques. Two photon microscopy
Author to be Deconvolution of wide-field and confocal images
announced
C O N F O C A L M I C R O S C O P Y -
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L A B O R A T O R Y S E S S I O N S and T U T O R I A L S
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1. Introduction to confocal imaging
2. Confocal imaging of live cells
3. 3-D image reconstruction
4. Ratio imaging
5. Image analysis
6. Novel multi-photon and time-resolved fluorescence techniques in
microscopy (tutorial)
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I acknowledge with gratitude the financial assistance of the Course co-
organizers and MAJOR SPONSORS:
INTERNATIONAL SOCIETY FOR ANALYTICAL CYTOLOGY (ISAC)
BIO-RAD
BECTON-DICKINSON
PARTEC
I am also grateful to the following Institutions for extending their help:
ATM
AutoQuant
Coulter
Equimed
Molecular Probes
Sigma-Aldrich
Verity Software House
and
the Jagiellonian University
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* REGISTRATION
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Register on-line via our WWW page:
http://helios.mol.uj.edu.pl/kurs/app.html
or write to: dobrucki@mol.mol.uj.edu.pl
* FURTHER INFORMATION:
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dr Jerzy Dobrucki
fax. 48 12 / 33 69 07
tel. 48 12 / 34-14-42, ex. 268
48 12 / 34-16-80, ex. 268
address:
Jagiellonian University
Institute of Molecular Biology
Al. Mickiewicza 3
31-120 Krak=F3w
Poland
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web