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I am attempting to measure apoptosis and necrosis within a population
of vascular smooth muscle cells which have been isolated from a rat
aorta.The cells were isolated by a 12hr collagenase/elastase
digestion an then used immediately for a two colour annexin V vs PI
experiment. The results are quite variable within the triplicates of each
experiment , which is quite worrying. The conditions such as cell number
incubation times etc. are the same for each experiment.
Any suggestions to improve the variability??
Thanks
James S. Clark PhD.
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web