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These ovarian tumor cells exclude PI, and have 'normal' forward vs side
scatter. Other PI+ cells in the mixture stain for cytokeratin, as do some
other PI negative cells that clearly have reduced forward scatter. I
assume both these two latter populations are nonviable or nearly so, and
might be nonspecifically staining with the anticytokeratin reagent. While i
understand PI exclusion is not foolproof for 'cell death', the absence of
PI staining and appropriate light scatter make me wonder about the
possibility of real cytokeratin expression on the cell surface of a
minority of these cells. My idea at this time is to sort out the cells and
see if they grow and maintain this surface expression. Any other
suggestions, experience or ideas?
thankyou
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Dr. Frederic I. Preffer
preffer@helix.mgh.harvard.edu
Department of Pathology- Warren 525A
100 Blossom St
Massachusetts General Hospital
Boston MA 02114
v(617) 726-7481 fax(617) 724-3164
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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