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I want to show by FACS that a protein is cell
surface expressed. In western blotting reduction
is required for detction of this epitope tag,
indicating Ab inaccessiblity in 3D structure. Are
there any methods to partially disrupt cell
surface proteins, and hence detect this epitope,
while maintaining cell viablility for FACS ?
Any info would be greatly appreciated.
Thanks in advance.
=05=05=05 Andy Riddell
PNAC Division
MRC Laboratory of Molecular Biology,
Hills Road
Cambridge CB2 2QH
U.K.
tel: (01223) 402 218
fax: (01223) 412 178
e-mail: ar3@mrc-lmb.cam.ac.uk
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web