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I am responding via the list because your e-mail system bounced back my
letter. I'm glad that the gentle ethanol fixation protocol described by
us a few years ago worked; we've used it for a number of applications to
"walk the line" between the need for good cell permeablization for cell
cycle analysis and preservation of surface markers for immunophenotyping.
Let me know if you need any additional assistance/advise.
Bill
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web