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I have an investigator who wants to look at intracellular distribution of a
drug using confocal laser scanning microscopy. The drug is not fluorescent,
so he has a monoclonal antibody (FITC conjugate) to the drug. Our question
is, during permeablization to allow the antibody into the cells, what is the
best way to prevent the drug from leaching out of the cells? I suppose a
pre-permeablization paraformaldehyde fixation step would be helpful, but I
would like to hear from anyone who has done such drug studies and knows if
this is an effective approach.
Thanks in advance.
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web