Green HeNe Laser - TexasRed/Cy3
O. Joseph Trask, Jr. (cytometry.osu.edu@postbox.acs.ohio-state.edu)
Tue, 10 Jun 1997 10:36:22 -0400 (EDT)
Greetings -
Is anyone out there using a Green NeHe Laser (~543nm) to excite Texas Red or
Cy3 for any type of immunophenotyping analysis, if so, how much output
power? Melles Griot has a 2mW Green NeHe Laser (543nm), but is 2mW output
power enough for immunofluorescence? I know 50mW from the 568nm line on a
Krypton Laser works well. All comments will be appreciated.
Regards,
O. Joseph Trask, Jr.
The Ohio State University
Comprehensive Cancer Center
Analytical Cytometry Laboratory
Tel: 614/292-FLOW(3569)
Fax: 614/292-7335
E-Mail: cytometry@osu.edu
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
