Identification of Eosinophils in Flow Cytometry

Hoffmann, H. J. (hjh@post1.tele.dk)
Tue, 17 Jun 1997 14:41:35 +0000

We work (almost) excliusively with human Eosinophils, and analyse
them on our Coulter Epics Profile II. Mostly, we purify them and do
not need to be able to identify them amongst other cells on a FS-SS
plot, which is difficult on our machiine, as they lie in the same
region as neutrophils.

I have recently dealt with someone that runs a BD FACS, and claims to
be able to identify Eosinophils as a population discrete from
neutrophils.

As we are considering purchase of a new flow cytometer, I would like
to invite comments on the identification of eosinophils on different
flow cytometers.

thanks very much!
hjh
Hans Juergen Hoffmann - Lung Research Clinic
Aarhus University Hospital, Denmark

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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu