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Both of these papers are up-to-date on the subject (e.g., ref.2 appearing
just this week in Blood) and neither of them utilizes analysis of CD45
expression to define the stem cell population.
Kevin G. Waddick, Ph.D.
Asst. Professor
University of Minnesota
Biotherapy Institute
2625 Patton Road
Roseville, Minnesota 55113
(612) 627-1920 ext. 131 Office
(612) 627-1928 Fax
On Fri, 6 Dec 1996, Glenda, M, Davison wrote:
>
>
> Greetings from Cape Town,
>
> I wonder if anyone out there can help me. I would like to set up a
> protocol for accurately quantitating CD34+ stem cells in peripheral
> blood stem cell harvests. It seems that most centres are dual
> labelling with CD45, but I would like some advice on which protocol
> to use as this seems to vary.
>
> I would be very grateful for any advice or any references I could use.
>
> Many thanks in advance,
>
> Glenda Davison
> Dept. Haematology
> Groote Schuur Hospital
> University of Cape Town
> email: gmd@samiot.uct.ac.za
>
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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