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I have not tried using saponin for permeablization but will be comparing
BD's FACSPerm to Caltag's Fix N' Perm.
If you would like more details, please email me directly.
-- Michelle
===========================================================================
Michelle N. Fiordalisi, Ph.D.
Clinical Immunology Fellow
University of North Carolina Hospitals
email: mnfiord@med.unc.edu
Phone: 919-966-4058 FAX: 919-966-0486
===========================================================================
On Thu, 20 Feb 1997, Vishal Gulati wrote:
>
>
> Hi! Has anyone on the list used BD Fastimmune Intracellular cytokine
> staining reagents. I have been using 'conventional' indirectly lablled
> cytokine antibodies and am planning to switch over.
>
> Also, has someone compared saponin with BD/any other commercial
> permeablising reagent .
>
> Id be grateful if those of you out here in the know of things share their
> experiences.
>
> Thanks in advance
>
>
> Bye
>
>
> Vishal
>
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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