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Gerhard.nebe-von-caron@unilever.com
______________________________ Reply Separator _________________________________
Subject: Absolute counts from flow cytometry
Author: Jeff_Carrell@hgsi.com at INTERNET
Date: 15/11/96 04:01
We are trying to generate absolute counts for different cell types in the
peripheral blood of mice. What are your preferred methods?
I understand that one common practice is to multiply the WBC (from an automated
cell counter such as a Coulter Counter) by the percent staining (from FACS).
However, we are questioning the validity of this approach because of the
different lysing methods used for the two types of instruments. Some non-
erythrocytes may be lysed in different proportions with each method. Is
multiplying this WBC count by a relative percentage accurate?
Of course, these instruments were designed for human blood, so trying to adapt
procedures for mice is a challenge...but it shouldn't be impossible. Any
thoughts would be greatly appreciated.
Jeff Carrell
Human Genome Sciences
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
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If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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