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Robin Barclay wrote:
> We want to tag free DNA to follow its possible delivery into cells
May I suggest that you look at the following paper:
Flow cytometric quantification of electroporation-mediated uptake of
macromolecules into plant protoplasts.
Blackhall N.W., Finch R.P., Power J.B., Cocking E.C. and Davey M.R.
Protoplasma (1995) 186:50-56
This paper looks at the uptake of FITC-Dextran and uses PI to assess
viability. In this work we were able to correlate dextran uptake
with transient gene expression following uptake using the same
parameters.
Please contact me if you need further assistance
Nigel Blackhall
Plant Genetic Manipulation Group,
Department of Life Science,
University of Nottingham, University Park, Nottingham NG7 2RD
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web