Many of these slides have been published in several of our Purdue Cytometry CDROM and DVD series, and may be used personally without copyright restriction provided you give credit to the author and reference this site. Commercial or for-profit use is strictly forbidden.
New EV detection technology www.celltient.com
Professors:
JPR: Dr. J. Paul Robinson, Purdue University Cytometry Laboratories
BR: Dr. Bartek Rajwa, PUCL
SK: Steve Kelley PUCL
WT: Dr. William Telford, Flow Lab at Hospital for Special Surgery, New York, NY
CCS: Dr. Carleton C. Stewart, (Retired)
SJS: Sigrid J. Stewart, (Retired)
BHD: Bruce Davis
Techniques for Giving a Great Seminar
This lecture will be helpful to students, postdocs and faculty and is focused on presenting that special seminar where you want to really communicate your message in a very professional way. This presentation shows you what to do and what not to do to get your message across.
Here is the Powerpoint file you can download.
Lectures:
| # | Title | Prof | Size | Link |
|---|---|---|---|---|
| Presentation on CD4 low cost tests in Africa | JPR | Listen | ||
| Presentation 101 for Graduate Students | JPR | Page | ||
Microscopy Series - BMS 524, "Introduction to Confocal Microscopy and Image Analysis." | ||||
| 1 | The Principles of Microscopy Part 1 [49 slides] | JPR | 6.153 MB | PPT |
| 2 | The Principles of Microscopy Part 2 [41 slides] | JPR | 2.714 MB | PPT |
| 3 | The Principles of Microscopy Part 3 | JPR | PPT | |
| 3a | Image Formats and Image Manipulations [29 slides] | SK | 5.794 MB | PPT |
| 4a | The Principles of Microscopy Part 3 [31 slides] | JPR | 4.265 MB | PPT |
| 5 | Fluorescence and Fluorescent Probes [52 slides] | JPR | 7.46 MB | PPT |
| 6 | The Principles of Confocal Microscopy [44 slides] | BR | 5.022 MB | PPT |
| 7 | 3D Imaging, Rotations and Stereo Imaging [33 slides] | JPR | 3.163 MB | PPT |
| 8 | Principles of 2D image analysis - Morphometry (I) [46 slides] | JPR | 1.623 MB | PPT |
| 9 | Applications of Morphometry (II) [36 slides] | JPR | 9.237 MB | PPT |
| 10 | Live Cell Imaging Application in Confocal Microscopy [38 slides] | JPR | 9.545 MB | PPT |
| 11 | Applications of Confocal Microscopy [24 slides] | JPR | 2.349 MB | PPT |
| 12 | Sample Preparation of Confocal Microscopy [25 slides] | JPR | 8.4 MB | PPT |
| 13 | Additional Applications of Confocal Microscopy [25 slides] | BR | PPT | |
Flow Cytometry Series - BMS 631, Slides are based on Howard Shaprio's great text "Practical Flow Cytometry" and many have page numbers to reference the material in that text. | ||||
| A | Introductory flow cytometry: Part 1- E-lecture - automated online lecture | JPR | 63 Mins | Listen |
| B | Introductory flow cytometry: Part 2- E-lecture - automated online lecture | JPR | 60 Mins | Listen |
| Fluorescence Quantitation - An overview of the salient issues including QC | JPR | 15.4 MB | PPT | |
| 1 | BMS 631 - LECTURE 1 Flow Cytometry: Theory (2006) | JPR | 28.8 MB | PPT |
| 2 | BMS 631 Flow Cytometry: Theory - Lecture 2 Whos and whys of flow cytometry - History(2006) | JPR | 8.32 MB | PPT |
| 3 | BMS 602A/631 - Lecture 3: : Light and Matter (2006) | JPR | 0.68 MB | PPT |
| 4 | BMS 602A/631: Lecture 4 : Light and Fluorescence (2005) | JPR | 0.51 MB | PPT |
| 5 | BMS 631 - Lecture 5: Properties and Applications of Light Sources (2005) | JPR | 3.34 MB | PPT |
| 6 | BMS 631 - Lecture 6: Optical Systems (2005) | JPR | 24.53 MB | PPT |
| 7 | BMS 631 - Lecture 7: Optics - Filter Properties and manipulation of light in flow cytometry (2005) | JPR | 0.17 MB | PPT |
| 8 | BMS 631: Lecture 8: Detectors (2004) | JPR | 2.8 MB | PPT |
| BMS 631 - Lecture: Flow systems and hydrodynamics (2005) | JPR | 8.41 MB | PPT | |
| 10 | BMS 631 - Lecture 10: Multiparameter data analysis (2004) | JPR | 1.71 MB | PPT |
These lectures are undated, but many have been here for many years, so they may not be as up updated as you might like. | ||||
| 1 | Introduction to Flow Cytometry - 1 [28 slides] | JPR | 5.3 MB | PPT Page |
| 2 | Introduction to Flow Cytometry -2 [32 slides] | JPR | 1.05 MB | PPT Page |
| 3 | Flow Cytometry Lecture 3 - Light and Matter [28 slides] | JPR | 213 KB | PPT Page |
| 4 | Flow Cytometry lecture 4 - Optical Systems [29 slides] | JPR | 1.94 MB | PPT Page |
| 5 | Flow Cytometry Lecture 5 - Light Sources and Optical Systems [26 slides] | JPR | 3.9 MB | PPT Page |
| 6 | Flow Cytometry Lecture 6 - Optics: Filter Properties [41 slides] Page | JPR | 235 KB | PPT Page |
| 7 | Flow Cytometry Lecture 7 - Detectors & Fluidics [54 slides] | JPR | 9.7 MB | PPT Page |
| 8 | Flow cytometry lecture 8 - Electronic measurements & Signal Processing [24 slides] | JPR | 95 KB | PPT Page |
| 9 | Flow Cytometry Lecture 9 - Data Collection: Linear, Log, Ratios... [24 slides] | JPR | 292 KB | PPT Page |
Older General Flow Cytometry Lectures | ||||
| Med 101 [59 slides] (4/5/2005) | JPR | PPT | ||
| Basic flow cytometry theory [17 slides] | JPR | Page | ||
| DNA analysis [28 slides] | JPR | Page | ||
| Multiple Color Analysis [3 slides] | WT | Page | ||
| Light Scatter [2 slides] | WT | Page | ||
| Excitation / Emission peaks [9 slides] | WT | Page | ||
| Applications [14 slides] | JPR | Page | ||
| Antibody Binding [49 slides] | CCS | Page | ||
| Multiparameter Immunofluorescence [67 slides] | CCS & SJS | Page | ||
| Compensation [16 slides] | CCS & SJS | Page | ||
| Simultaneous Measurement of Necrotic, Apoptotic Dead and Live Cells [4 slides] | CCS | Page | ||
| Immunophenotyping Leukemias & Lymphomas [23 slides] | CCS | Page | ||
| Breast Cancer: DNA Content & Relevant Markers [4 slides] | CCS | Page | ||
| Automated Reticulocyte Analysis [27 slides] | BHD | Page | ||
| Immunophenotyping of Hematopoietic Progenitor Cells by Multiparameter Flow Cytometry [20 slides] | CCS | Page | ||
