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______________________________ Reply Separator _________________________________
Subject: Sorting Bacteria
Author: BarrenP@MedImmune.com at INTERNET
Date: 05/04/97 05:22
Flowers!
Does anyone have any experience with sorting bacteria (Streptococcus)
on a stream in air flow cytometer. I am using a
FACSTAR Plus.
What I think I need is a dye that will allow me to counter stain all
the bacteria (preferably live) to be used in conjunction with a dim
surface label, and then sort on the surface labeled positives. The
counter stain would need to be dim so as not to interfere with the dim
surface label.
I have tried to sort with a SSC log threshold. and this does not work.
As soon as I vibrate the stream I loose all low end resolution which
is where my bacteria are.
I need to use a fluorescent trigger to threshold on, this would be less
sensitive to stream vibrations which is what I assume is killing me on
the log SSC threshold.
Just a note all my Scatter parameters are on logarithmic amplification.
Thanks in advance for your time and energy
Philip Barren
Pb
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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