Methods for sorting viable X and Y sperm
CHELACK@admin3.usask.ca
Mon, 16 Jun 1997 11:56:27 -0600 (CST)
What kind of methods are being used to sort viable X and sperm in particular
bull sperm. A graduate student has brought me some older literature
describing sorting on differences in DNA content using specialized adaptors
to maintain consistent orientation of the sperm as they pass through the flow
cell. Is it possible to use FITC oligos to specific regions of the Y
chromosome to accomplish this? I have sent the graduate student back to search
out more on this possibility, but am wondering if anyone out there is already
doing this or has found it to be impossible, or is using another even more
elegant method? The graduate student is quite keen and quite green so I am
trying to make sure he isn't biting off more than he can chew.
regards
BJC - Brian
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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