DNA stain

Gabriel Alespeiti (galespei@nybc.org)
Fri, 13 Jun 1997 20:00:36 -0400

Dear flowers:

We have to check the ploidy on megakaryocytes. The cells have to be viable
after staining. We cannot use Hoechst 33342 because our flow cytometer does
not have a UV laser. Is there any other dye that we can use for this purpose?

Thanks,

Gabriel E. Alespeiti
Hematopoitic Growth Factors Lab
New York Blood Center
310 E.67th Street
New York, NY 10021
Phone: (212) 570-3115
Fax: (212) 570-3195
E-mail:galespei@nybc.org


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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu